human il-4 elispot pro kit (alp)  (Mabtech Inc)

Journal: Cell

Article Title: Protective prototype-Beta and Delta-Omicron chimeric RBD-dimer vaccines against SARS-CoV-2

doi: 10.1016/j.cell.2022.04.029

Figure Lengend Snippet: Immunogenicity of prototype-Beta chimeric vaccine in rhesus macaques Twenty-four rhesus macaques (see Table S1 ) were immunized with 3 doses of prototype-Beta chimeric RBD-dimer vaccine or sham (n = 12), 21 days apart. Serum samples were collected according to the study schedule shown in Figure S5 B. (A) Endpoint titer of antigen-binding IgG. (B) 50% neutralization titer of pseudotyped virus (prototype, Alpha, Beta, Delta, and Omicron) in serum. (C) 50% neutralization titer of authentic SARS-CoV-2 (prototype, Alpha, Beta, and Delta). (D) 50% neutralization titer of authentic SARS-CoV-2 (prototype, Beta and Omicron). The values shown in (A)–(D) are the GMT ± 95% confidence interval (CI). (E) Summed IFN-γ, IL-2 and IL-4 ELISPOT responses in PBMCs collected at day 6 after the third dose toward peptides spanning SARS-CoV-2 RBD. The values are means ± SEM. p values were analyzed with two-tailed Mann-Whitney test. ( ∗∗ p < 0.01; ∗∗∗ p <0.001; ∗∗∗∗ p < 0.0001).

Article Snippet: Human IL-4 ELISpot PRO kit (ALP) , MabTech , Cat#3410-2APW-10.

Techniques: Immunopeptidomics, Binding Assay, Neutralization, Virus, Enzyme-linked Immunospot, Two Tailed Test, MANN-WHITNEY

Journal: Cell

Article Title: Protective prototype-Beta and Delta-Omicron chimeric RBD-dimer vaccines against SARS-CoV-2

doi: 10.1016/j.cell.2022.04.029

Figure Lengend Snippet:

Article Snippet: Human IL-4 ELISpot PRO kit (ALP) , MabTech , Cat#3410-2APW-10.

Techniques: Virus, Isolation, Infection, Variant Assay, Recombinant, Adjuvant, Enzyme-linked Immunospot, Software, Microscopy